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Fig. 4 | BMC Plant Biology

Fig. 4

From: Molecular cloning and metabolomic characterization of the 5-enolpyruvylshikimate-3-phosphate synthase gene from Baphicacanthus cusia

Fig. 4

Functional characterization of BcEPSPS in vitro. a SDS-PAGE analysis of BcEPSPS purified by Ni-NTA conjugation. Lane 1, protein molecular weight marker; lane 2, crude enzyme extract from BcEPSPS-pET-32a cell lysate; lane 3, supernatant of pET-32a-BcEPSPS cell lysate; lane 4, purified pET-32a-BcEPSPS protein; lane 5, crude enzyme extract from pET-32a cell lysate. b Western blotting analyses of BcEPSPS. Lane 1, protein molecular weight marker; lane 2, supernatant of pET-32a-BcEPSPS cell lysate; lane 3, purified BcEPSPS protein; lane 4, purified pET-32a protein

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