Fig. 3

Site-specific PCR analyses of Mariam insertion A4–4 (see Additional file 1: Table S1). The PCR analysis was done using primers flanking the insertion in accessions collected from five populations of wild emmer wheat (Mt. Hermon, Amiad, Tabgha, Jaba, and Mt. Amasa) and in eight accessions of T. aestivum collected from different countries (1 – Georgia, 2 - Iran, 3 - USA, 4 – China, 5 - UK, 6 - Spain, 7 - Turkey, 8 – former Yugoslavia, see Additional file 2: Table S2). This insertion was polymorphic in all wild emmer wheat populations, while monomorphic (empty site) in all bread wheat accessions. Each accession presents either a 445 bp (full site) or a 149 bp band (empty site). M denotes a size marker (the numbers in the left are in bp), while NC denotes a negative control (water was used as template in PCR reaction)