Fig. 5

Analysis of vitamin E in leaves and grains of 637ys. Elution profiles of the tocopherol standards (a), tocopherols in leaves of wild-type ZH11 (b) and 637ys (c), tocopherols and tocotrienols in grains of ZH11 (d) and 637ys (e) were detected by fluorescence with excitation at 290 nm and emission at 330 nm. Tocopherol contents in leaves and grains of ZH11 and 637ys (f) were quantified by using tocopherol standards. α-T, α-tocopherol; γ-T, γ-tocopherol. The tocopherol standards were prepared by pooling identical volumes of α-tocopherol, γ-tocopherol and δ-tocopherol at the same concentration. Peaks 1 and 2 represent α-tocopherol and γ-tocopherol; Peak 3 is δ-tocopherol which does not exist in rice and was used as control. Peaks 4, 5 and 6 represent α-tocotrienol, γ-tocotrienol and δ-tocotrienol, respectively. Peak 7 might be the isomer of γ-tocopherol. Error bars represent standard errors of three independent biological replicates. Asterisks indicate statistically significant differences (with Student’s t test) compared with the wild-type at P < 0.01