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Table 2 Contents of free amino acids (mg/g fresh weight) in the leaves of tea plant treated with methionine sulfoximine (MSX). The value shown is mean ± standard deviation (SD). CK indicates plants grown with nutrient solution without MSX treated, while MSX indicate plants treated with MSX. The asterisk indicates significant difference between MSX and CK group (*P < 0.05, **P < 0.01 in student’s t-test). For each treatment 3 biological replicates were used

From: Short-term inhibition of glutamine synthetase leads to reprogramming of amino acid and lipid metabolism in roots and leaves of tea plant (Camellia sinensis L.)

 

CK

MSX

P (t-test)

Theanine

2.98 ± 0.06

3.92 ± 0.23

0.18

Glutamate

0.47 ± 0.04

0.40 ± 0.02

0.11

Valine

0.22 ± 0.01

0.16 ± 0.02*

0.02

Serine

0.21 ± 0.03

0.13 ± 0.01*

0.02

Aspartic acid

0.18 ± 0.01

0.13 ± 0.01*

0.03

Alanine

0.17 ± 0.01

0.11 ± 0.01**

0.0016

Arginine

0.16 ± 0.05

0.20 ± 0.01

0.83

Cysteine

0.12 ± 0.01

0.08 ± 0.01**

0.0047

Methionine

0.12 ± 0.01

0.10 ± 0.01

0.06

Glycine

0.12 ± 0.01

0.12 ± 0.02

0.48

Threonine

0.11 ± 0.01

0.08 ± 0.01*

0.03

Leucine

0.11 ± 0.01

0.06 ± 0.01*

0.02

Histidine

0.11 ± 0.01

0.13 ± 0.01

0.82

Tyrosine

0.07 ± 0.01

0.05 ± 0.01

0.06

Lysine

0.03 ± 0.01

0.02 ± 0.01*

0.03

Phenylalanine

0.02 ± 0.01

0.01 ± 0.01**

0.0003