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Table 2 Contents of free amino acids (mg/g fresh weight) in the leaves of tea plant treated with methionine sulfoximine (MSX). The value shown is mean ± standard deviation (SD). CK indicates plants grown with nutrient solution without MSX treated, while MSX indicate plants treated with MSX. The asterisk indicates significant difference between MSX and CK group (*P < 0.05, **P < 0.01 in student’s t-test). For each treatment 3 biological replicates were used

From: Short-term inhibition of glutamine synthetase leads to reprogramming of amino acid and lipid metabolism in roots and leaves of tea plant (Camellia sinensis L.)

  CK MSX P (t-test)
Theanine 2.98 ± 0.06 3.92 ± 0.23 0.18
Glutamate 0.47 ± 0.04 0.40 ± 0.02 0.11
Valine 0.22 ± 0.01 0.16 ± 0.02* 0.02
Serine 0.21 ± 0.03 0.13 ± 0.01* 0.02
Aspartic acid 0.18 ± 0.01 0.13 ± 0.01* 0.03
Alanine 0.17 ± 0.01 0.11 ± 0.01** 0.0016
Arginine 0.16 ± 0.05 0.20 ± 0.01 0.83
Cysteine 0.12 ± 0.01 0.08 ± 0.01** 0.0047
Methionine 0.12 ± 0.01 0.10 ± 0.01 0.06
Glycine 0.12 ± 0.01 0.12 ± 0.02 0.48
Threonine 0.11 ± 0.01 0.08 ± 0.01* 0.03
Leucine 0.11 ± 0.01 0.06 ± 0.01* 0.02
Histidine 0.11 ± 0.01 0.13 ± 0.01 0.82
Tyrosine 0.07 ± 0.01 0.05 ± 0.01 0.06
Lysine 0.03 ± 0.01 0.02 ± 0.01* 0.03
Phenylalanine 0.02 ± 0.01 0.01 ± 0.01** 0.0003