Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC domain are required for protein binding and when mutated lead to delayed peduncle and flag leaf senescence

Table 1 Selected TILLING mutations in NAM-A1

Line	SNP	RefSeqv1 chr6 (bp)	Consequence	Sub domain	Mutation	SIFT	Region [20]	Consequence in ANAC019
Kronos3661	C/T	77,100,016	Missense	i	P38S	0	Dimerisation	–
Kronos2615	G/A	77,100,006	Missense	i	R41Q	0	Dimerisation	Protein Binding [24]
Kronos2734	C/T	77,099,994	Missense	i	T45 M	0	Dimerisation	–
Kronos2711	C/T	77,099,581	Missense	iii	R110W	0	DNA interface	DNA Binding [21, 24]
Kronos2551	G/A	77,099,511	Missense	–	C133Y	0.78		–
Kronos2060	C/T	77,099,448	Missense	iv	P154L	0	DNA interface	–
Kronos3186	G/A	77,099,450	Synonymous	iv	P153P	NA		–
Kronos1107	G/A	77,099,212	Splice Acceptor Variant	v	SAV	NA	DNA interface	–

ISSN: 1471-2229