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Fig. 2 | BMC Plant Biology

Fig. 2

From: NAL8 encodes a prohibitin that contributes to leaf and spikelet development by regulating mitochondria and chloroplasts stability in rice

Fig. 2

Map-based cloning of NAL8 and genetic complementation. a The NAL8 locus was initially mapped to chromosome 7 between 07 and 30,610 and 07–52,402 marker loci, and was further delimited to a 72-kb region between marker loci NAL8-Q and RM5499 that contains 12 open reading frames. Numbers below the marker loci indicate the number of recombinants. The NAL8 gene is predicted to have five exons (blue boxes), and the green arrows indicate the direction of transcription on chromosome 7. The start (ATG) and stop (TGA) codons are indicated as well. A single nucleotide mutation (a G to A transition) in NAL8 causes a predicted alanine to threonine change at amino acid position 228. b The predicted protein structures of NAL8 and NAL8A228T determined by SWISS-MODEL. Arrows indicate the potential structural site changes in the homo-trimers. The table shows the protein quality estimates for the two predicted structures. c Amino acid sequence alignment of the partial SPFH domain in predicted prohibitin protein sequences from monocot and dicot species. The red arrow indicates the mutated site between TQ and the nal8 mutant, which is highly conserved in all examined plant prohibitins. d Plant architecture at the reproductive stage of the NAL8 transgenic complementation lines NAL8com#1 and #2 in the nal8 mutant background compared with TQ and the nal8 mutant Scale bar = 15 cm. e Leaves of TQ, nal8 and the transgenic complementation lines NALcom#1 and #2. Scale bar = 5 cm. f Statistical analysis of average flag leaf lengths in TQ, nal8 and the transgenic complementation lines NALcom#1 and #2. Values are given as the mean ± SD. *P < 0.05; **P < 0.01 compared with the corresponding TQ control using Student’s t-test in f

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