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Table 4 Changes of LN stress on metabolites content in old leaves of two wild soybean varieties

From: Physiological and metabolomics analyses of young and old leaves from wild and cultivated soybean seedlings under low-nitrogen conditions

Metabolite name Relative concentration Fold changes Log2(LN/CK)
C W
CK LN CK LN C W
Aliphatic
amino acid
Asparagine 0.51 ± 0.06 0.06 ± 0.02 0.06 ± 0.01 0.22 ± 0.05 −3.20** 1.95*
Aspartic acid 1.53 ± 0.33 0.79 ± 0.24 0.07 ± 0.04 0.33 ± 0.09 −0.95 2.28*
Threonine 1.43 ± 0.03 1.24 ± 0.15 0.02 ± 0.00 0.03 ± 0.01 −0.20 0.97*
Cycloleucine 0.85 ± 0.06 1.31 ± 0.55 30.89 ± 5.31 44.38 ± 8.78 0.63 0.52
Isoleucine 5.58 ± 0.20 2.58 ± 0.19 0.38 ± 0.01 1.33 ± 0.25 −1.11 1.79**
L − proline 2.46 ± 1.02 5.06 ± 0.68 0.11 ± 0.01 0.81 ± 0.18 1.04 2.85**
L − homoserine 0.29 ± 0.05 0.33 ± 0.02 1.04 ± 0.21 2.42 ± 0.26 0.19 1.22**
Valine 5.63 ± 0.87 4.42 ± 0.09 37.13 ± 3.86 47.46 ± 5.60 −0.35* 0.35
Alanine 2.46 ± 0.32 1.61 ± 0.52 0.09 ± 0.01 0.26 ± 0.03 −0.61 1.57**
Glycine 3.51 ± 0.54 2.62 ± 0.46 1.07 ± 0.15 1.27 ± 0.30 −0.42* 0.25
Serine 8.50 ± 0.84 12.51 ± 2.47 0.01 ± 0.00 0.06 ± 0.01 0.56 2.32*
Sarcosine 1.59 ± 0.17 2.07 ± 0.23 0.81 ± 0.15 2.97 ± 0.63 0.38 1.88*
Aromatic amino acid Phenylalanine 0.71 ± 0.21 0.49 ± 0.04 0.08 ± 0.03 0.17 ± 0.02 −0.53 1.01*
Non − protein nitrogen Galactosamine 0.08 ± 0.01 0.10 ± 0.00 9.79 ± 4.25 17.95 ± 3.79 0.26 0.87
GABA 8.34 ± 0.78 6.38 ± 1.42 0.10 ± 0.02 0.19 ± 0.02 −0.39 0.92*
Maleimide 0.93 ± 0.08 0.77 ± 0.18 0.23 ± 0.01 0.31 ± 1.10 −0.27 0.43*
Mannosamine 0.83 ± 0.09 1.06 ± 0.08 3.67 ± 0.66 10.39 ± 0.63 0.36 1.50**
Ethanolamine 19.62 ± 0.57 20.71 ± 4.45 30.85 ± 6.18 61.33 ± 6.31 0.08 0.99*
Sugars and polyols Glycerol 14.12 ± 0.18 11.49 ± 0.27 0.10 ± 0.01 0.20 ± 0.00 −0.30 0.95**
Monopalmitin 0.04 ± 0.01 0.16 ± 0.05 25.16 ± 8.47 106.06 ± 10.98 1.83 2.08
Myo − inositol 19.77 ± 0.48 28.56 ± 1.03 0.60 ± 0.07 1.48 ± 0.41 0.53** 1.31**
Palatinitol 0.04 ± 0.00 0.06 ± 0.00 0.29 ± 0.06 0.52 ± 0.11 0.78** 0.85
Arabitol 1.46 ± 0.14 1.57 ± 0.06 0.12 ± 0.01 0.57 ± 0.10 0.10 2.23**
Threitol 0.84 ± 0.23 1.25 ± 0.14 0.17 ± 0.01 0.37 ± 0.06 0.57 1.15*
Erythritol 0.08 ± 0.02 0.20 ± 0.03 1.23 ± 0.25 4.26 ± 0.15 1.24** 1.79*
Sucrose 36.97 ± 1.51 29.08 ± 1.92 30.81 ± 9.34 2.64 ± 0.02 −0.35** −3.54*
Maltose 3.03 ± 0.42 2.37 ± 0.32 0.12 ± 0.01 0.22 ± 0.05 −0.36 0.91
Melezitose 0.24 ± 0.11 0.23 ± 0.02 0.004 ± 0.00 0.03 ± 0.00 −0.11 2.77**
L − threose 0.12 ± 0.03 0.12 ± 0.01 4.15 ± 0.23 4.96 ± 0.75 −0.04 0.26
Fucose 0.17 ± 0.06 0.10 ± 0.00 0.08 ± 0.00 0.12 ± 0.01 −0.81 0.68**
Xylose 0.22 ± 0.03 0.37 ± 0.03 6.36 ± 0.17 10.71 ± 2.81 0.75* 0.75
Galactose 0.87 ± 0.20 1.70 ± 0.15 0.05 ± 0.01 0.08 ± 0.01 0.96* 0.53
Fructose-6 − biphosphate 0.06 ± 0.01 0.22 ± 0.04 0.06 ± 0.00 0.15 ± 0.01 1.83** 1.25**
Glucose−6 − phosphate 0.03 ± 0.00 0.14 ± 0.04 0.95 ± 0.15 1.55 ± 0.22 2.49* 0.70
Organic acid Lactic acid 2.17 ± 0.21 4.79 ± 0.91 0.69 ± 0.09 0.93 ± 0.17 1.15* 0.44
Galactonic acid 0.63 ± 0.08 2.08 ± 0.25 1.05 ± 0.35 1.42 ± 0.14 1.72** 0.44
Glyceric acid 8.33 ± 0.94 8.12 ± 2.57 9.87 ± 0.91 15.71 ± 2.56 −0.04 0.67*
Pyruvic acid 7.98 ± 0.17 2.84 ± 0.71 0.34 ± 0.08 0.85 ± 0.11 −1.49** 1.31*
Fumaric acid 44.89 ± 4.92 7.64 ± 1.23 0.06 ± 0.02 0.07 ± 0.01 −2.55** 0.11
Malic acid 0.16 ± 0.01 0.11 ± 0.01 3.39 ± 0.57 5.29 ± 1.30 −0.50* 0.64
Citramalic acid 0.35 ± 0.06 0.23 ± 0.02 0.16 ± 0.00 0.26 ± 0.04 −0.62 0.71*
Succinic acid 7.73 ± 0.72 7.15 ± 1.28 6.33 ± 1.30 10.06 ± 0.74 −0.11 0.67*
Ketoisocaproic acid 0.02 ± 0.00 0.03 ± 0.00 11.15 ± 0.46 22.50 ± 0.38 0.55** 1.01**
2 − Deoxytetronic acid 18.32 ± 3.67 30.44 ± 2.30 3.47 ± 0.61 9.71 ± 1.54 0.73 1.49
Oxalic acid 0.19 ± 0.03 0.24 ± 0.04 0.12 ± 0.01 0.26 ± 0.04 0.71 2.26*
Propionic acid 0.14 ± 0.03 0.17 ± 0.01 0.83 ± 0.20 3.03 ± 0.34 0.34 1.13**
Maleic acid 14.74 ± 1.66 7.75 ± 1.14 1.73 ± 0.20 9.20 ± 3.54 0.28* 1.86
Gluconic acid 0.11 ± 0.01 0.12 ± 0.02 0.10 ± 1.03 0.34 ± 0.09 −1.10 0.58*
Glucoheptonic acid 1.98 ± 0.13 2.64 ± 0.20 1.71 ± 0.49 3.57 ± 0.71 −0.93 2.41
Fatty acids Butyrate 0.31 ± 0.01 0.15 ± 0.07 25.66 ± 1.03 38.35 ± 2.81 0.13 1.83*
Isobutyric acid 0.26 ± 0.05 0.43 ± 0.04 2.53 ± 0.38 12.14 ± 3.61 0.41 1.06
Stearic acid 0.16 ± 0.01 0.42 ± 0.07 1.92 ± 0.10 3.50 ± 0.53 1.43* 0.87*
Linoleic acid 0.01 ± 0.00 0.02 ± 0.00 8.30 ± 2.49 22.03 ± 0.87 0.83* 1.41**
Linolenic acid 0.34 ± 0.03 1.14 ± 0.16 3.19 ± 0.40 2.49 ± 0.33 1.75** −0.36
Palmitic acid 0.55 ± 0.04 1.64 ± 0.22 0.27 ± 0.02 0.82 ± 0.26 1.56** 1.59
Butyric acid 0.31 ± 0.22 0.14 ± 0.01 0.95 ± 0.10 0.67 ± 0.04 −1.09 −0.52*
Phenylpropanoids Neohesperidin 0.81 ± 0.11 2.14 ± 0.45 0.17 ± 0.03 1.27 ± 0.24 1.40* 2.90**
Salicylic acid 1.23 ± 0.00 1.60 ± 0.17 2.28 ± 0.24 7.70 ± 1.42 0.38 1.76**
Trans − cinnamate 0.36 ± 0.03 1.08 ± 0.20 1.43 ± 0.11 4.59 ± 0.74 1.59* 1.68**
Naringin 0.01 ± 0.00 0.06 ± 0.02 0.78 ± 0.24 1.81 ± 0.59 3.04* 1.21
Ferulic acid 0.81 ± 0.08 1.16 ± 0.05 0.47 ± 0.04 0.31 ± 0.03 0.52* −0.61**
Caffeic acid 0.01 ± 0.00 0.02 ± 0.00 6.48 ± 0.68 5.12 ± 0.54 1.16 −0.34
Prunin 0.29 ± 0.02 0.19 ± 0.07 2.41 ± 0.51 5.61 ± 0.19 −0.61* 1.22*
  1. Relative concentrations and standard deviation were increased by a factor of 100 times in each treatment. Values were presented as the mean ± standard error of four biological replicates. C Cultivar soybean, W Wild soybean, CK Control treatment, LN Low−N stress; * and ** indicate significant (P < 0.05) and highly significant (P < 0.01) differences, respectively