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Fig. 1 | BMC Plant Biology

Fig. 1

From: A fern WUSCHEL-RELATED HOMEOBOX gene functions in both gametophyte and sporophyte generations

Fig. 1

Expression of CrWOXB in the wild-type gametophyte localized to regions of cellular proliferation. a RT-PCR of CrWOXB expression in gametophytes; d8, d13, 8- and 13-days post-plating, respectively. CrUBQ used as control. (b-m) In-situ hybridization of whole-mount emerging (b, c), 8-day (d, e), 10-day (f, g) and 13-day (h, i) hermaphroditic gametophytes; (c, e, g, i) are sense controls, black and red arrows (b-i) indicate the notch-meristem regions and archegonia, respectively. Insets show staining in whole gametophytes. (j-m) In-situ hybridization of male gametophtyes before (j, k) and after (l, m) antheridium differentiation; (k, m) are sense controls, arrows in (j) depict differentiated antheridia. Scale bar = 0.05 mm

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