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Fig. 4 | BMC Plant Biology

Fig. 4

From: Transcriptomic and phosphoproteomic profiling and metabolite analyses reveal the mechanism of NaHCO3-induced organic acid secretion in grapevine roots

Fig. 4

Functional categories (a) of DEGs responding to alkali stress and the key genes and proteins involved in the metabolism and extrusion of organic acid (b, c) and signaling related to ethylene, IAA and receptor kinases (c). RNA-Seq was performed using the top 10-mm length of grapevine roots after 12 h of NaHCO3 treatment. In panel a, functional categories of the DEGs from RNA-Seq were performed by Gene Ontology. In panel b, the schematic was constructed using the genes and proteins related to organic acid metabolism and extrusion. The normalized values of gene expression are shown on a color scale, and a P highlighted with a yellow background color denotes a phosphoprotein. The gray color indicates the possible transporters of oxalate. In panel c, the heatmap was constructed using the genes related to organic acid metabolism and transport, metabolism and signaling of ethylene and IAA, and receptor kinases. The normalized values of gene expression are shown on a color scale. Fold changes of qRT-PCR were calculated by comparing the relative expression values of the selected genes in the NaHCO3-treated and control vine roots

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