Fig. 5

Trehalose, ROS (H₂O₂), and sucrose contents in wild-type (WT) and mutant/OE lines. a Trehalose contents in the whole seedlings of WT, tppf1 mutant, and OE9 lines under control and drought-stress conditions. Error bars indicate SEs (n = 9). Different lowercase letters (a and b or c and d) indicate significant differences among lines (Duncan’s test; p < 0.05). b Relative abundance of AtTPPF transcripts in REST (the rest part of the whole seedlings apart from the SAM) and SAM (shoot apical meristem) of WT plants under control and drought conditions. Error bars indicate SEs (n = 4). Different lowercase letters (a and b or c and d) indicate significant differences among lines (Duncan’s test; p < 0.05). c Trehalose contents in SAM and REST under control and drought conditions. Error bars indicate SEs (n = 3). Different lowercase letters (a and b or c and d) indicate significant differences among lines (Duncan’s test; p < 0.05). d H₂O₂ contents in 10-d-old seedlings on MS treated without (Control) or with PEG6000 (Drought) for 24 h and subjected to DAB staining. Three biological repeats were analyzed. Black arrows indicate new leaves. e Soluble sugars contents in 10-d-old WT, tppf1 mutant, and OE9 plants with or without drought treatment. Error bars show SEs (n = 4). Different lowercase letters (a, b, or c) indicate significant differences among lines (Duncan’s test, p < 0.05). f Sucrose contents in 10-d-old WT, tppf1 mutant, and OE9 plants with or without drought treatment. Error bars indicate SEs (n = 4). Different lowercase letters (a, b, c, d or e) indicate significant differences among lines (Duncan’s test; p < 0.05)