Fig. 2

Overexpression of AtTPPF improves drought tolerance of Arabidopsis thaliana. a Schematic diagram of overexpression construct of AtTPPF. b RT-PCR analysis of AtTPPF gene transcripts in wild-type (WT) and three AtTPPF-overexpressing lines (OE6, OE5, and OE9). eIF-4A gene was used as internal control. c Analysis of drought-tolerance phenotypes of three overexpression lines in (b). Six containers of each genotype (nine plants per container) of 10-d-old plants growing in soil were subjected to drought by withholding water for 26 d. Three independent assays were performed, each producing similar results. d Plant survival rates at 2 d after rewatering. Plants in (c) were re-watered, and survival rates were determined by counting surviving plants after 2 d. Three independent experiments were performed, each containing approximately 81 plants. Data are means ± SEs. Different lowercase letters indicate significant differences among lines (Duncan’s test; p < 0.05)