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Fig. 7 | BMC Plant Biology

Fig. 7

From: Cyclic nucleotide-gated ion channel 6 mediates thermotolerance in Arabidopsis seedlings by regulating nitric oxide production via cytosolic calcium ions

Fig. 7

Patch-clamp analysis of Ca2+-permeable channels in wild-type, cngc6, noa1, cngc6/35S::NOA1–1, and cngc6/35S::NIA2–1 seedlings. The Ca2+ current before HS (at 22 °C, control) and after HS (at 37 °C, HS) was compared in the root cell protoplasts of 10-day-old wild-type (a), cngc6 (b), noa1 (c), cngc6/35S::NOA1–1 (d) and cngc6/35S::NIA2–1 (e) plants. The Ca2+ current was recorded by step voltage clamp. Each trace is a representative current from six protoplasts. Currents in the protoplasts are shown in the left and middle columns, respectively. The I–V curve is shown in the right column (mean ± SD, n = 6)

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