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Fig. 4 | BMC Plant Biology

Fig. 4

From: Reconstruction of the full-length transcriptome atlas using PacBio Iso-Seq provides insight into the alternative splicing in Gossypium australe

Fig. 4

RT-PCR validation of AS events for ten genes. cDNA from 5 h, 15 h and 30 h germinating seeds and leaf samples were used for RT-PCR. Primer sets flanking the splicing events (F, forward and R, reverse) were designed. The gel bands in each figure show DNA markers and the RT-PCR results from four tissues/samples. The splice structure of each gene is shown in the right panel. Black boxes show exons, and lines show introns. The expected sizes are shown beside the structures. Asterisk indicates the isoform did not appear in Iso-Seq

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