Fig. 2

AS analysis of G. australe with Iso-Seq reads. a Visualization of five AS types. b The total number of AS events in G. australe based on Iso-Seq data. The number and percentage of AS events are shown at the top of each bar. c Distribution of genes that produce one or more splice isoforms in the Iso-Seq data. The number and percentage of genes are shown in the top of each bar. d Sashimi plot showing an example of a gene generating various transcript isoforms detected with our pipeline in the Iso-Seq data. There are nine different isoforms, including nine splice junction sites in Iso-Seq. Peaks in red represent short-read coverage, and curved lines with numbers in red represent splice junctions supported by that number of short reads. For each isoform, the blocks in blue represent exons, and the lines between the blocks represent introns