Reconstruction of G. australe FL transcript loci from Iso-Seq. a Pipeline used for reconstruction of FL transcript loci from Iso-Seq using a combination of the ‘has genome’ and ‘no genome’ strategies. b Functional annotation against various databases. Protein databases including Swiss-Prot UniProt (UniProt), Ensembl Plants (Plants) and NCBI NR (NR) were used for BLASTp annotation, and Pfam (Pfam) was used for Pfam Scan annotation. The number and percentage of common annotated genes are shown in the overlapping segment of the Venn diagrams. c Numbers of different transcript loci detected in the FL transcriptomes using the combination strategy. Cupcake, ‘G. raimondii genome as reference’; Cogent, ‘reconstructed gene sequences as reference’.