Phenotypic and genotypic characterization of near-isogenic lines targeting a major 4BL QTL responsible for pre-harvest sprouting in wheat

Table 1 Sprouting and germination tests of four parents and eight confirmed pairs of NILs for QPhs.ocs-4B.1 conferring PHS resistance in wheat

Number	Parents/NILs	Genetic background	SP (%)	Difference in SP (%)	GI	Difference in GI (%)
1	Chara (Non-dormant, PHS susceptible parent)		68.26		0.63
2	Westonia (Non-dormant, PHS susceptible parent)		83.40		0.74
3	DM5637B*8 (Dormant, PHS resistant parent)		14.85		0.18
4	SUN326AE (Dormant, PHS resistant parent)		19.31		0.22
5	NIL_PHSR4BL_1R	SUN326AE × Westonia F8	21.44	70.86**	0.33	59.41**
6	NIL_PHSR4BL_1S		73.56		0.81
7	NIL_PHSR4BL_2R	SUN326AE × Westonia F8	33.60	54.01**	0.29	61.56*
8	NIL_PHSR4BL_2S		73.05		0.76
9	NIL_PHSR4BL_3R	Chara × DM5637B*8 F8	13.69	76.75**	0.69	10.84
10	NIL_PHSR4BL_3S		58.88		0.77
11	NIL_PHSR4BL_4R	Chara × DM5637B*8 F8	16.19	69.61**	0.19	70.52*
12	NIL_PHSR4BL_4S		53.28		0.64
13	NIL_PHSR4BL_5R	Chara × DM5637B*8 F8	14.71	77.60*	0.22	69.54**
14	NIL_PHSR4BL_5S		65.65		0.72
15	NIL_PHSR4BL_6R	Chara × DM5637B*8 F8	21.18	58.90*	0.67	8.79
16	NIL_PHSR4BL_6S		51.53		0.73
17	NIL_PHSR4BL_7R	Chara × DM5637B*8 F8	7.11	81.88**	0.19	70.11*
18	NIL_PHSR4BL_7S		39.22		0.65
19	NIL_PHSR4BL_8R	Chara × DM5637B*8 F8	20.14	64.44*	0.67	6.31
20	NIL_PHSR4BL_8S		56.63		0.72

‘R’ lines are those with an allele from the resistant parent (SUN326AE or DM5637B*8), and ‘S’ lines are those with an allele from the susceptible parent (Westonia or Chara); * and ** represent significant differences at p < 0.05 and p < 0.01 levels, respectively; ‘SP’ is sprouting percentage; ‘GI’ is germination index

ISSN: 1471-2229