Fig. 6

Altered sensitivity to Pi-deficiency in GmPHO1 transgenic Arabidopsis. a, b 35S::GmPHO1;H4 transgenic lines were grown on normal (1.25 mM Pi) (a) and -Pi (0 mM Pi) medium (b) for 2 weeks. c, d 35S::GmPHO1;H6 transgenic lines were grown on normal (1.25 mM Pi) (c) and -Pi (0 mM Pi) medium (d) for 2 weeks. The red dashed line indicates the root length of the WT. Bar = 1 cm. e-p Expression of marker genes involved in the Pi pathways in transgenic Arabidopsis. The expression of PHO1;H1, AT4, IPS1, and PHT1;4 in transgenic Arabidopsis plants harboring GmPHO1;H4 (e-h), GmPHO1;H8 (i-l), and GmPHO1;H6 (m-p), respectively. Wild-type (WT) and transgenic lines grew in normal or -Pi MS medium for 2 weeks. Expression of each gene in the WT (blue column) under normal conditions was set as 1. The black stars (* or **) indicates the difference significance relative to the gene expression in WT under normal conditions, whereas the red ones indicate a significant difference relative to the gene expression in the WT under Pi-deficiency. The * means statistically significant difference at the P < 0.05 level, and ** indicates statistically significant difference at the P < 0.01 level