Fig. 9

AtPARP2 is poly (ADP-ribosyl) ated by AtPARP1 in vivo. a AtPARP2 is poly (ADP-ribosyl) ated in the presence of supplemented NAD⁺ under zeocin treatment. b The poly (ADP-ribosyl) ation of AtPARP2 accumulated in the parg1 mutant and diminished in the parp1 mutant. The 10-d-old seedlings were treated with 200 μg/mL zeocin for 48 h. The total proteins were extracted with or without exogenously supplemented 1 mM NAD⁺, and PAR signals were detected using anti-pan-ADPR reagent. AtPARP2 was detected with an anti-AtPARP2 antibody. The immunoblot results with anti-tubulin antibody or the Coomassie blue-stained Rubisco large unit (Rbc L) band were used to show the protein loading amounts. Red lines indicate the poly (ADP-ribosyl) ated AtPARP2. TUB, tubulin