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Fig. 7 | BMC Plant Biology

Fig. 7

From: New perspectives on the plant PARP family: Arabidopsis PARP3 is inactive, and PARP1 exhibits predominant poly (ADP-ribose) polymerase activity in response to DNA damage

Fig. 7

AtPARP1 produces most of the PAR signal under zeocin and MMS treatments. a Time-dependent PAR signal generated by wild-type seedlings after zeocin treatment at 100 μg/mL. b Time-dependent PAR signal generated by wild-type seedlings after zeocin treatment at 200 μg/mL. c Time-dependent PAR signal generated by wild-type seedlings after MMS treatment at 100 μg/mL. d Time-dependent PAR signal generated by wild-type seedlings after MMS treatment at 200 μg/mL. e PAR signal generated by different mutants of AtPARP1 and AtPARP2 after zeocin treatment at 100 μg/mL for 24 h. f PAR signal generated by different mutants of AtPARP1 and AtPARP2 after MMS treatment at 200 μg/mL for 24 h. g Time-dependent induction of AtPARP2 by zeocin treatment. PAR signal was detected using anti-pan-ADPR reagent. AtPARP2 was detected with an anti-AtPARP2 antibody. Tubulin was detected using an anti-tubulin antibody to show the protein loading amounts. Red arrows indicate the poly (ADP-ribosyl) ated AtPARP1. Yellow arrows indicate the poly (ADP-ribosyl) ated AtPARP2. TUB, tubulin

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BMC Plant Biology

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