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Fig. 6 | BMC Plant Biology

Fig. 6

From: New perspectives on the plant PARP family: Arabidopsis PARP3 is inactive, and PARP1 exhibits predominant poly (ADP-ribose) polymerase activity in response to DNA damage

Fig. 6

AtPARP3 does not show poly (ADP-ribose) polymerase activity in seeds. a PAR signal generated in wild-type and mutant seeds in the presence of broken DNA and NAD+. b The PARP inhibitor 3-AB can eliminate the PAR signal. Fifty milligrams of wild-type or various mutant seeds was incubated in distilled water for 24 h. Total proteins were extracted from the seeds and then incubated with 1 mM NAD+ in the presence of 500 nM broken DNA for 10 min at 25 °C. PAR signals were detected using anti-pan-ADPR reagent. Anti-tubulin antibody was used in the immunoblot assay to show the protein loading amounts. p1 p2, parp1–3 parp2–3; p1 p3, parp1–1 parp3–1; p2 p3, parp2–1 parp3–1; p1 p2p3, parp1–3 parp2–3 parp3–1; and TUB, tubulin

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