Fig. 2From: New perspectives on the plant PARP family: Arabidopsis PARP3 is inactive, and PARP1 exhibits predominant poly (ADP-ribose) polymerase activity in response to DNA damageActivity determination of recombinant proteins with domain swapping. a Schematic diagram of the domain swapping experiments between AtPARP1 and AtPARP3. Different boxes show different domains. b Activity determination of the domain-exchanged proteins. The purified proteins were incubated with 500 nM broken DNA and 1 mM NAD+ at 25 °C for different time periods with or without 20 mM 3-AB and then detected by immunoblotting using different antibodies. The arrows in the Coomassie blue-stained SDS-PAGE gel indicate the recombinant proteins TRXH-AtPARP1 (red), TRXH-AtPARP3 (green), TRXH-AtP1-P3 (orange), and TRXH-AtP3-P1 (black). Anti-His antibody was used to detect the expressed TRXH-AtP1-P3 and TRXH-AtP3-P1 proteinsBack to article page