Table 1 The primer sets used in this study
Primer Set | Upstream (5′-3′) | Downstream (5′-3′) | Primer Anneal Temperature (°C) | Fragment Size (bp) |
|---|---|---|---|---|
TaAFP-AF1/R1 | GATTCTACTGGCTCTGCTT | GAGCAAGGAAGCGCAATAG | 57 | 808 |
TaAFP-AF2/R2 | CCGTCGTCGACCAGGGGAA | CAGACTCTGACGCTACGTAACTC | 63 | 568 |
TaAFP-AF3/R3 | GAGATGCCTTCTACGGCATG | GAGGAAGTTGCCGTGGCAA | 60 | 613 |
TaAFP-BF1/R1 | GATTCTGCTGGCTCTGCTT | CAAGGGAACGCAATAGAAC | 57 | 828 |
TaAFP-BF2/R2 | CCGTCGTCGACCAGGGGAA | ACCCTGACGCTACGTAACTGT | 62 | 587 |
TaAFP-BF3/R3 | GAGATGCCTTCTACGGCATG | CCCCCTAGGTACATTCCGA | 59 | 733 |
TaAFP-DF1/R1 | GATTGTGCTGGCTCTGCTT | TTGGAGTAGCAGGGAAGCT | 57 | 770 |
TaAFP-DF2/R2 | CCGTCGTCGACCAGGGGAA | TCCCTCCGTTCCAAAATAGATGACT | 62 | 618 |
TaAFP-DF3/R3 | GAGATGCCTTCTACGGCATG | ACGATGTGCCTGAGCGGA | 60 | 706 |
AFPBF/R | CTTCCTGAGAATTTGGCCGT | TGAGCTCGACCACCTCGTCG | 61 | 207,203 |
ACTINF/R | GTTTCCTGGAATTGCTGATCGCAT | CATTATTTCATACAGCAGGCAAGC | 62 | 410 |
Q-TaAFP-BF/R | ACCTCCTCAAGCATGCCGG | ACTTGTTCTGGTTGCTGGCA | 66 | 102 |
Q-TaAFP-AF/R | CCCTCCTCAAGCATGCCCG | GCTGGCATTG TTATTATTATCG | 59 | 100 |
Q-TaAFP-DF/R | GCTTCCTCAAGCATGCCGGA | CGTCCACCTTGGAGGAGACT | 56 | 289 |
Q-ABI5F/R | GGAAGAAGTCACCTCRCACC | GAGGCAAGGAGAACGACT | 62 | 310 |
Pro F/R | GATTCTGCTGGCTCTGCTT | ACGGCCAAATTCTCAGGAAG | 59 | 152 |