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Table 6 QTL for TOF in alfalfa identified in a pseudo-testcross F1 (3010 x CW 1010) population. The phenotypic data was assessed for two years for TOF in two locations (JPC and BVL)

From: QTL mapping of flowering time and biomass yield in tetraploid alfalfa (Medicago sativa L.)

Parent

QTL code

Chr.

Location/Year

Peak Marker

Peak LOD

R2

Allele Dir.

LSI (cM)

Flanking Markers

3010

Tof-d1

1D

JPC/ Π

MRG_14469273

4.4

0.08

–

34.9–36.9

TP56677 - TP66486

3010

Tof-d2

1D

JPC/Π

TP60376

3.3

0.06

–

30.6–31.1

TP60376 - TP41436

3010

Tof-d3

3C

JPC/Π

TP52465

3.5

0.06

+

38.1–44.0

TP37583 - TP72054

3010

Tof-d4

7A

JPC/Π

TP58371

3.3

0.06

+

27.3–30.4

TP58371 - TP2134

3010

Tof-d5

7A

JPC/Π

TP24733

3.8

0.07

+

37.5–38.7

TP55743 - TP34483

3010

Tof-d6

1A

JPC/β

TP85729

3.3

0.06

+

95.1–96.6

TP5699 - TP36877

3010

Tof-d7

3B

JPC/β

TP68861

7.7

0.15

–

42.5–45.9

TP60221 - TP68861

3010

Tof-d8

7A

JPC/β

TP28256

5.1

0.09

+

2.4–6.1

TP28256 - TP80202

3010

Tof-d9

7B

JPC/β

TP3421

4.8

0.09

+

23–27.2

TP9376 - TP3421

3010

Tof-d10

1A

BVL/Π

TP35274

3.3

0.07

+

88.9–91.3

TP52576 - TP995

3010

Tof-d11

1B

BVL/Π

TP23433

3.8

0.09

–

45–49.7

TP23433 - TP66714

3010

Tof-d12

3D

BVL/Π

TP18933

3.4

0.07

–

19.3–24.4

MRG_22559848 - TP66479

3010

Tof-d13

6D

BVL/β

MRG_2402742

3.7

0.08

+

16.2–23.0

TP16313 - TP18699

CW 1010

Tof-n1

5B

JPC/Π

TP11856

4.1

0.07

–

75.3–79.4

TP80448 - TP80460

CW 1010

Tof-n2

6B

JPC/Π

TP3310

7.1

0.14

–

17.2–21.2

TP3310 - TP71145

CW 1010

Tof-n3

6D

JPC/Π

TP48161

4.8

0.09

+

54.8–56.4

MRG_5981048 - TP6188

CW 1010

Tof-n4

6D

JPC/Π

TP49028

5.5

0.10

+

60.1–60.5

TP70280 - TP66860

CW 1010

Tof-n5

6D

JPC/Π, BVL/ β

TP24444

3.8

0.08

+

65.9–66.8

TP64001- TP32647

CW 1010

Tof-n6

7C

JPC/Π, JPC/β

TP44666

8.0

0.16

–

42.9–44.7

TP45002 - TP4972

CW 1010

Tof-n7

7C

JPC/Π, JPC/β

TP54614

4.1

0.07

–

48.4–54.7

TP38417 - TP54614

CW 1010

Tof-n8

7B

JPC/β

TP9019

4.0

0.07

–

23.7–26.1

TP14107 - MRG_9345022

CW 1010

Tof-n9

7B

JPC/β

TP36500

4.7

0.09

–

30.8–31.6

TP36500 - MRG_25777286

CW 1010

Tof-n10

8B

BVL/Π

TP76596

3.6

0.08

–

19.3–31.5

TP76596 - TP75547

CW 1010

Tof-n11

8B

BVL/Π

TP75547

3.1

0.07

–

31.5–38.9

TP75547 - TP25170

CW 1010

Tof-n12

4B

BVL/ β

TP66329

4.6

0.09

–

5.5–12.8

TP88701 - TP57672

  1. JPC and BVL refer to the two locations - the J. Phil Campbell Sr. Research and Education Center (JPC) in Watkinsville and the Georgia Mountain Research and Education Center (BVL) in Blairsville, GA, respectively
  2. The negative allele direction (−) refers to the shorter days to flowering or the QTL responsible for early flowering, and the positive allele direction (+) refers to the QTL responsible for late flowering. The peak and flanking markers given are based on 1- LOD support interval (LSI)
  3. Π Year 2015, β Year 2017, Chr. Chromosome, Dir. Direction, LSI 1-LOD support interval in cM unit