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Table 6 QTL for TOF in alfalfa identified in a pseudo-testcross F1 (3010 x CW 1010) population. The phenotypic data was assessed for two years for TOF in two locations (JPC and BVL)

From: QTL mapping of flowering time and biomass yield in tetraploid alfalfa (Medicago sativa L.)

Parent QTL code Chr. Location/Year Peak Marker Peak LOD R2 Allele Dir. LSI (cM) Flanking Markers
3010 Tof-d1 1D JPC/ Π MRG_14469273 4.4 0.08 34.9–36.9 TP56677 - TP66486
3010 Tof-d2 1D JPC/Π TP60376 3.3 0.06 30.6–31.1 TP60376 - TP41436
3010 Tof-d3 3C JPC/Π TP52465 3.5 0.06 + 38.1–44.0 TP37583 - TP72054
3010 Tof-d4 7A JPC/Π TP58371 3.3 0.06 + 27.3–30.4 TP58371 - TP2134
3010 Tof-d5 7A JPC/Π TP24733 3.8 0.07 + 37.5–38.7 TP55743 - TP34483
3010 Tof-d6 1A JPC/β TP85729 3.3 0.06 + 95.1–96.6 TP5699 - TP36877
3010 Tof-d7 3B JPC/β TP68861 7.7 0.15 42.5–45.9 TP60221 - TP68861
3010 Tof-d8 7A JPC/β TP28256 5.1 0.09 + 2.4–6.1 TP28256 - TP80202
3010 Tof-d9 7B JPC/β TP3421 4.8 0.09 + 23–27.2 TP9376 - TP3421
3010 Tof-d10 1A BVL/Π TP35274 3.3 0.07 + 88.9–91.3 TP52576 - TP995
3010 Tof-d11 1B BVL/Π TP23433 3.8 0.09 45–49.7 TP23433 - TP66714
3010 Tof-d12 3D BVL/Π TP18933 3.4 0.07 19.3–24.4 MRG_22559848 - TP66479
3010 Tof-d13 6D BVL/β MRG_2402742 3.7 0.08 + 16.2–23.0 TP16313 - TP18699
CW 1010 Tof-n1 5B JPC/Π TP11856 4.1 0.07 75.3–79.4 TP80448 - TP80460
CW 1010 Tof-n2 6B JPC/Π TP3310 7.1 0.14 17.2–21.2 TP3310 - TP71145
CW 1010 Tof-n3 6D JPC/Π TP48161 4.8 0.09 + 54.8–56.4 MRG_5981048 - TP6188
CW 1010 Tof-n4 6D JPC/Π TP49028 5.5 0.10 + 60.1–60.5 TP70280 - TP66860
CW 1010 Tof-n5 6D JPC/Π, BVL/ β TP24444 3.8 0.08 + 65.9–66.8 TP64001- TP32647
CW 1010 Tof-n6 7C JPC/Π, JPC/β TP44666 8.0 0.16 42.9–44.7 TP45002 - TP4972
CW 1010 Tof-n7 7C JPC/Π, JPC/β TP54614 4.1 0.07 48.4–54.7 TP38417 - TP54614
CW 1010 Tof-n8 7B JPC/β TP9019 4.0 0.07 23.7–26.1 TP14107 - MRG_9345022
CW 1010 Tof-n9 7B JPC/β TP36500 4.7 0.09 30.8–31.6 TP36500 - MRG_25777286
CW 1010 Tof-n10 8B BVL/Π TP76596 3.6 0.08 19.3–31.5 TP76596 - TP75547
CW 1010 Tof-n11 8B BVL/Π TP75547 3.1 0.07 31.5–38.9 TP75547 - TP25170
CW 1010 Tof-n12 4B BVL/ β TP66329 4.6 0.09 5.5–12.8 TP88701 - TP57672
  1. JPC and BVL refer to the two locations - the J. Phil Campbell Sr. Research and Education Center (JPC) in Watkinsville and the Georgia Mountain Research and Education Center (BVL) in Blairsville, GA, respectively
  2. The negative allele direction (−) refers to the shorter days to flowering or the QTL responsible for early flowering, and the positive allele direction (+) refers to the QTL responsible for late flowering. The peak and flanking markers given are based on 1- LOD support interval (LSI)
  3. Π Year 2015, β Year 2017, Chr. Chromosome, Dir. Direction, LSI 1-LOD support interval in cM unit