Fig. 5
From: GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton
Loss of GhYGL1d expression affects chloroplast development. a-h Chlorophyll fluorescence and morphology of the protoplasts isolated from the CLCrVA (a, e), GhYGL1d-RNAi-1(b, f), GhYGL1d-RNAi-2 (c, g) and GhYGL1d-RNAi-3 (d, h) plant leaves. (i-l) Transmission electron micrographs of plastid ultrastructures in the CLCrVA (I), GhYGL1d-RNAi-1(J), GhYGL1d-RNAi-2 (K) and GhYGL1d-RNAi-3 (L) plants. Plastids were from leaves of six-week-old plants. Three biological replicates were performed, and similar results were obtained. SG, starch grain. Thy, thylakoid. Gr, granum. Bars = 1 μm.