Fig. 4

Confirmation of the expression patterns of the DECs by semiquantitative RT-PCR and qRT-PCR a Semiquantitative RT-PCR shows that the expression patterns of seven upregulated circRNAs and one downregulated circRNA. n = 3 replicates. (b-h) The expression pattern of seven upregulated circRNAs and (i) one downregulated circRNA confirmed by qRT-PCR. A heatmap of each circRNA was generated from the normalized transcripts per million (TPM) value and used to visualize the circRNA expression level in the RNA-sequencing data. OsActin1 was used as a reference gene. The error bars indicate standard deviation of three replicates. Asterisks indicate a significant difference as determined by Student’s t-test (*P < 0.05; **P < 0.01)