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Fig. 4 | BMC Plant Biology

Fig. 4

From: The differential response of cold-experienced Arabidopsis thaliana to larval herbivory benefits an insect generalist, but not a specialist

Fig. 4

Previous cold treatment alters the transcriptional response to herbivory and artificial wounding. Leaves of A. thaliana were untreated (C2), cold-treated (P2), damaged by Pieris brassicae (TP) or Mamestra brassicae feeding (TM) or artificial wounding (TW), or cold-treated followed by feeding/wounding damage (P + TP, P + TM and P + TW). N = 3 biological replicates of each sample type. a Principle component analysis (PCA) of the normalized gene expression of individual experimental leaf samples. The first two components, which explain most of the changes, are depicted (explained variances are shown at the axes). Ellipses indicate the 95% confidence interval. b Venn diagrams of genes regulated in response to larval feeding or artificial wounding with and without prior cold treatment. Blue characters, genes specifically regulated upon cold treatment; green characters, genes specifically regulated upon damage; red characters, genes regulated upon both cold per se and damage per se; orange characters, genes regulated only when the plant had been exposed to the combination of prior cold and subsequent damage; colored intersections, genes that were differentially regulated in P + T plants relative to T plants (P + T vs T) and also in untreated (T vs C2) or cold-treated, damaged plants (P + T vs C2) relative to control plants. c Venn diagram with the genes in the colored sectors in panel (b). d Numbers of differentially expressed genes (DEGs) in cold-treated and herbivore- or wounding-damaged plants compared to untreated plants (P + T vs C2; left panel) and in cold-treated herbivore- or wounding-damaged plants compared to untreated, damaged plants (P + T vs T; right panel). e Genes responsive to herbivory or artificial wounding with enhanced or attenuated expression changes in cold-treated relative to untreated plants

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