Fig. 4

Expression profiles of GhZFP genes during fiber development. a RNA-seq data heat map of GhZFP gene expression levels during different stages of fiber growth. The differences in gene expression are shown in different colors. b Cis-element analysis of GhZFP8 promoters. E-box (CANNTG) motifs (red) were predicted using the online toolkit, PLACE. A total of 2,000 bp promoter sequence for each GhZFP8 were used for the analysis. c Exogenous BR promotes the transcription of GhZFP8. Statistical significance was determined using one-way analysis of variance combined with Tukey’s test. *P < 0.05; **P < 0.01; ***P < 0.001