Fig. 4

ATG8 directly interacts with NbHYPK. a GFP labelled NbHYPK, NbHYPKΔUBA and UBA-NbHYPK was co-infiltrated with RFP-labelled ATG8 and localized by confocal microscopy. b Bimolecular fluorescence complementation (BiFC) analysis, ATG8-Yn or x-Yn(empty vector) was co-expressed with NbHYPK-Yc, NbHYPKΔUBA-Yc and UBA-NbHYPK-Yc or x-Yc (empty vector). Fluorescent signals were visualized after 3 dpi by confocal microscopy. Bar = 1 μm. c Analysis of interaction between ATG8 and NbHYPK by Yeast two hybrid assay. Plasmids expression full length NbHYPK or the UBA domain or NbHYPKΔUBA or empty vector were transformed into AH109 yeast and mated with yeast expressing plasmids containing ATG8 or an empty vector and grown in amino acid drop out YPDA media