Table 3 The primers for qRT-PCR
Primers | Sequence (5′ → 3′) | Tm (°C) | PCR products |
|---|---|---|---|
PhTPS1-Q5′ | AGTTTCCGTTTGTGTGGGTG | 58.0 | 132 bp |
PhTPS1-Q3′ | CCGTTGTAGTAGAGGTGGGC |  | |
PhTPS2-Q5′ | TGCTGGGGGTGGAAGGG | 59.0 | 196 bp |
PhTPS2-Q3′ | GGGGAAGGGGGTGTGGAG |  | |
PhTPS3-Q5′ | CTGCCCACTCGTTTTCCA | 57.0 | 142 bp |
PhTPS3-Q3′ | CCGGCTCAATTTCTTCCAG |  | |
PhTPS4-Q5′ | TGTATGATGGGGACCGAACG | 58.0 | 184 bp |
PhTPS4-Q3′ | GCCACGGAATGTGAAGGAAG |  | |
Ph18S-Q5′ | AGTTAGGGGATCGAAGACGA | 55.0 | 153 bp |
Ph18S-Q3′ | CAGCCTTGCGACCATACTC |  |