Fig. 5

Validation of DEGs by qRT-PCR. The white bar and grey bar represent the fold change of the relative expression level from the RNA-seq data and qRT-PCR data, respectively. The wheat Actin gene, as an internal control, was used to normalize the expression levels of the target genes. The error bars represent the standard deviations of three replicates. L6vsL0: flag leaf samples under C6 compared with C0; T6vsT0: stem samples under C6 compared with C0; S6vsS0: seed samples under C6 compared with C0