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Fig. 5 | BMC Plant Biology

Fig. 5

From: Comparative secretome analysis of different smut fungi and identification of plant cell death-inducing secreted proteins from Tilletia horrida

Fig. 5

Functional validation of signal peptides (SPs) of putative T. horrida effectors using a yeast invertase secretion assay. The SPs predicted two T. horrida putative effectors. All transformed yeast strains YTK12 were able to grow on YPRAA media with raffinose as the sole carbon source (1% yeast extract, 2% peptone, 2% raffinose, and 2 μg of antimycin A per liter). The N-terminal sequences of Phytophthora sojae Avr1b and Magnaporthe oryzae Mg87 were used as positive and negative controls, respectively. The untransformed YTK12 did not grow on either CMD-W (0.67% yeast N base without amino acids, 0.075% tryptophan dropout supplement, 2% sucrose, 0.1% glucose, and 2% agar) or YPRAA media. Yeast growth on CMD-W media was equally viable among the transformed strains. Row a: CMD-W media; row b: YPRAA media; Mg87 (−): negative controls Mg87 SPs; Avr1b (+):positive controls Avr1b SPs; sp2965: SPs of smut_2965; sp5844: SPs of smut_5844

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