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Fig. 3 | BMC Plant Biology

Fig. 3

From: Comparative secretome analysis of different smut fungi and identification of plant cell death-inducing secreted proteins from Tilletia horrida

Fig. 3

The putative effectors in T. horrida induce necrotic cell-death phenotypes in Nicotiana benthamiana and treated leaves were stained with Trypan blue. a The symptoms of N. benthamiana leaves after inoculation with smut_2965 and smut_5844. PMDC32-GFP means PMDC32 vector containing the green fluorescent protein (GFP) gene; the same labeling was used for PMDC32-BAX, PMDC32–2965, and PMDC32–5844. Numbers, e.g., 23/27, indicate that 23 of 27 infiltrated leaves exhibited cell death or mottling phenotypes. Agrobacterium carrying green fluorescent protein (GFP) served as a negative control. Agrobacterium carrying the BAX vector served as a positive control. Representative photos were taken four days after infiltration. b The expressed proteins were detected in the leaves of N. benthamiana by western blot experiments. M: marker; B1–4: smut_2965 (13.86 kDa), smut_5844 (24.84 kDa), BAX (21 kDa), and GFP (27 kDa). c The phylogeny of smut_2965 and 12 fungi homology secreted proteins. The phylogeny was constructed using Mega v7.0.26. tca denotes Tilletia caries, tco denotes Tilletia controversa, tin denotes Tilletia indica, twa denotes Tilletia walkeri, tho denotes Tilletia horrida, uho denotes Ustilago hordei, uma denotes Ustilago maydis, src denotes Sporisorium reilianum, ssc denotes Sporisorium scitamineum, lbi denotes Laccaria bicolor, sco denotes Schizophyllum commune, mgr denotes Magnaporthe grisea, uvi denotes Ustilaginoidea virens, and rso denotes Rhizoctonia solani

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