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Fig. 10 | BMC Plant Biology

Fig. 10

From: Comparative secretome analysis of different smut fungi and identification of plant cell death-inducing secreted proteins from Tilletia horrida

Fig. 10

Yeast two-hybrid analysis of the smut_5844. a-b Autoactivation activity test of smut_5844 protein in yeast. a: SD-Trp/−Leu, b: SD-Trp/−Leu/−His/−Ade/X-α-gal. pAD-WT/pBD-WT was used as positive controls, and pAD-WT/pLaminC was used as a negative control. WT, Wild-type fragment C of lambda cI repressor (aa 132–236); Mut, E233K mutated fragment of lambda cl repressor (aa 132–236); Lamin C, human lamin C (aa 67–230). c Interaction between smut_5844 and OsLAC10 in a yeast two-hybrid system. P53 and SV40 were used as a pair of positive control. Lam and SV40 were used as a negative control. SD-2, SD-Trp-Leu; SD-4, SD-Trp-Leu-His-Ade. d The expression patterns of OsLAC10 and OsRbs5 in resistant and susceptible rice male sterile lines after T. horrida inoculation

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