Table 2 Performance of Spodoptera littoralis larvae on leaves from conventional and ULGCS cotton. First instars were fed with leaf discs from conventional cotton (Coker 312) and two ULGCS cotton lines (RNAi 1: 66-49B and RNAi 2: 66–274) for 7 days. Survival, weight, and consumed leaf area were measured after four and 7 days. Four larvae received leaf material from one plant and were pooled for analyses, 20 plants were tested per treatment (n = 20)
From: Constitutive and induced insect resistance in RNAi-mediated ultra-low gossypol cottonseed cotton
Cotton type | Four days | Seven days | ||||
|---|---|---|---|---|---|---|
Weight (mg ± SE) | Leaf area consumed (cm2 ± SE) | Survival (Number larvae ± SE) | Weight (mg ± SE) | Leaf area consumed (cm2 ± SE) | Survival (Number larvae ± SE) | |
Untreated cotton plants | ||||||
Coker 312 | 3.96 ± 0.260 | 0.90 ± 0.062 | 3.29 ± 0.171 | 36.64 ± 3.653 | 5.14 ± 0.435 | 3.19 ± 0.178 |
RNAi 1 | 4.14 ± 0.337 | 1.12 ± 0.094 | 3.27 ± 0.150 | 35.78 ± 3.101 | 5.33 ± 0.336 | 3.14 ± 0.190 |
RNAi 2 | 4.01 ± 0.218 | 0.94 ± 0.056 | 3.27 ± 0.199 | 34.04 ± 2.832 | 5.03 ± 0.267 | 3.14 ± 0.190 |
Spodoptera littoralis damaged cotton plants | ||||||
Coker 312 | 1.29 ± 0.164 | 0.40 ± 0.058 | 3.40 ± 0.240 | 6.38 ± 0.882 | 1.18 ± 0.158 | 3.15 ± 0.261 |
RNAi 1 | 2.05 ± 0.239 | 0.70 ± 0.081 | 3.40 ± 0.152 | 9.98 ± 1.726 | 1.82 ± 0.308 | 3.25 ± 0.160 |
RNAi 2 | 1.96 ± 0.246 | 0.64 ± 0.081 | 3.55 ± 0.135 | 12.24 ± 1.806 | 1.86 ± 0.254 | 3.25 ± 0.160 |
Jasmonic acid treated cotton plants | ||||||
Coker 312 | 2.37 ± 0.159 | 0.74 ± 0.058 | 3.70 ± 0.128 | 21.75 ± 2.585 | 3.38 ± 0.302 | 3.55 ± 0.135 |
RNAi 1 | 2.44 ± 0.169 | 0.80 ± 0.068 | 3.35 ± 0.196 | 19.94 ± 1.940 | 3.16 ± 0.315 | 3.30 ± 0.193 |
RNAi 2 | 2.72 ± 0.232 | 0.78 ± 0.069 | 3.05 ± 0.235 | 19.92 ± 2.565 | 3.22 ± 0.392 | 2.95 ± 0.211 |