Fig. 8

OsNHX5 regulates the pH of Golgi, TGN and PVC. a In vivo calibration curve of pH. pH calibration was achieved by equilibrating intracellular pH with 10 μM nigericin, 60 mM KCl, and 10 mM MES/HEPES Bis-Tris-propane, pH 5.0 to 8.0. (mean ± SD; n ≥ 25 protoplasts). b and c Representative pseudocolored images of PRpHluorin-BP80 (Y612A) in wild-type (b) or gpa6 (c) protoplasts. Bars = 5 μm. d pH of the Golgi, TGN and PVC. (mean ± SD; n ≥ 25 protoplasts; **P < 0.01; Student’s t test)