Fig. 6

Phylogenetic analyses and spatial expression patterns of OsNHX5. a A neighbor-joining tree of OsNHX5 and its homologs. The tree was constructed using MEGA and bootstrapped with 1000 replicates. b Wild-type expression levels of OsNHX5 and OsNHX6 in various organs and different developmental stages of endosperm. R, root; S, stem; L, leaf; LS, leaf sheath; P, panicle; DAF, days after flowering. Actin1 was used as an internal control. For each RNA sample, three technical replicates were performed. Values are means ± SD