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Fig. 3 | BMC Plant Biology

Fig. 3

From: Expression of AtLEC2 and AtIPTs promotes embryogenic callus formation and shoot regeneration in tobacco

Fig. 3

Shoot regeneration from callus derived from different transgenic lines. a-c Embryogenic callus separated from SAM of 20 DAG seedlings under 20 μM DEX induction. Scale bars = 3 mm. d-f 4 d after embryogenic callus on hormone-free MS medium (no DEX). Scale bars = 3 mm. g-i 12 d after embryogenic callus on hormone-free MS medium (no DEX). Scale bars = 3 mm. j-l 20 d after embryogenic callus on hormone-free MS medium (no DEX). Scale bars = 3 mm. m Statistics of shoot regeneration number of embryogenic callus on hormone-free MS medium (no DEX). Cyan indicated embryogenic callus derived from three independent AtLEC2-GR transgenic lines (each line contains 20 replicates). Dark blue indicated embryogenic callus derived from three independent AtIPT7-OE AtLEC2-GR transgenic lines (line 9, 10 and 11, and each line contains 20 replicates). Yellow indicated embryogenic callus derived from three independent AtIPT9-OE AtLEC2-GR transgenic lines (line 8, 9 and 12, and each line contains 20 replicates). Values are mean ± standard errors form 60 replicates. Three asterisks indicate statistically significant differences (***P < 0.001, Student’s t-test). d, days after transferred to hormone-free MS medium

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