Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean

Table 1 Summary of T0 genotyping data for GmFAD2–1A and GmFAD2–1B

^aMutant allele(s) was not transferred to progenies
^bAt least one mutant allele was transferred to progenies
^cTwo mutant alleles were stably transferred to progenies; the 3rd allele (−4 in Fig. 4c) was not inherited and likely an artifact of sequencing
^dInheritance of mutant alleles were not determined

ISSN: 1471-2229