Fig. 5

LsVe1L specific PCR assay is allele-specific. Shown are results of LsVe1L-specific PCR assays with selected lettuce accessions with known LsVe genotypes and resistance phenotypes. Resistance (R) and susceptibility (S) is indicated by capital letters for each accession. In all cases, the outcomes of the PCR assays were as expected from genome sequencing. Amplicon sizes are indicated by > and correspond to 200 and 500 bp. Lane numbers are: 1. 2-log ladder, 2. cultivar Balady Banha (Ve genotype: LsVe1L, LsVe3L, LsVe4L), 3. cultivar Lolla Rossa (LsVe1L, LsVe3L, LsVe4L), 4. cultivar Plymouth (LsVe1L, LsVe3L, LsVe4L), 5. cultivar Cobham Green (LsVe3L, LsVe4L, LsVe1S, LsVe2S), 6. cultivar Lee Tal (LsVe4L, LsVe1S, LsVe2S), 7. cultivar Margarita (LsVe4L, LsVe1S, LsVe2S), 8. cultivar Anuenue (LsVe2S, LsVe3S), 9. cultivar Blonde Lente a Monter (LsVe1S, LsVe2S, LsVe3S), 10. cultivar Primus (LsVe1S, LsVe2S, LsVe3S), 11. negative control, and 12. 2-log ladder. PCR conditions are described in Table 4