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Table 2 Identification of the main POD inhibitors from Excoecaria leaves by UPLC-DAD-QTOF-MS/MS

From: In planta high levels of hydrolysable tannins inhibit peroxidase mediated anthocyanin degradation and maintain abaxially red leaves of Excoecaria Cochinchinensis

Fractiona Compound No.b Rt(min) Molecular Formulac Molecular Mass mass (ppm) m/z Putative Namec
[M-H] Fragment Ions
F1 1 0.509 C13 H16 O10 332.0754 3.05 331.0682 151.0030; 169.0135; 211.0233; 331.0616 Galloyl hexoside [49] (Monogalloylglucose)
2 0.726 C13 H16 O10 332.0745 0.59 331.0673 151.0032; 169.0135; 211.0235; 331.0642 Galloyl hexoside [49] (Monogalloylglucose)
F2 3 0.517 C14 H16 O10 344.0748 1.41 343.0676 125.0250; 169.0137; 191.0552; 343.0642 Galloyl quinic acid [49]
4 0.859 C14 H16 O10 344.0749 1.49 343.0676 127.0398; 169.0136; 191.0549; 343.0642 Galloyl quinic acid [49]
F13 5 1.629 C8 H8 O5 184.0375 1.99 183.0303 78.0112;124.0168; 153.0219; 168.0070 Methyl gallic acid
6 1.724 C41 H28 O27 952.0814 0.38 951.0738 300.9991; 461.0359; 583.0310; 933.0622 Ellagitannin (HHDP derivative [49])
7 1.855 C55 H28 O26 1104.0936 0.76 1103.0859 300.9992; 445.0416; 631.0566; 951.0722 Ellagitannin [50] (HHDP derivative)
8 2.002 C56 H30 O26 1118.1099 6.57 1117.1012 166.9995; 300.9995; 461.0346; 965.0902 Ellagitannin [50] (HHDP derivative)
F14 9 1.608 C8 H8 O5 184.0373 0.5 183.0560 78.0109;124.0164; 153.0175; 168.0058 Methyl gallic acid
10 1.612 C41 H28 O27 952.0819 0.16 951.0747 169.0143; 300.9993; 461.0390; 933.0627 Ellagitannin (Galloyl HHDP derivative [49])
11 1.862 C42 H30 O27 966.097 0.51 965.0897 300.9994; 461.0401; 805.9860; 933.0648 Ellagitannin [50] (HHDP derivative)
12 1.885 C34 H28 O22 788.1076 0.42 787.1003 169.0143; 465.0666; 573.0867; 617.0789 Tetragalloyl hexoside (Tetragalloyl glucose) [49]
13 2.017 C41 H32 O26 940.1182 3.83 939.1110 125.0247; 169.0146; 465.0682; 617.0791 Pentagalloyl hexoside (Pentagalloyl glucose) [49]
  1. a The fractions were of high inhibitory efficacy on POD as indicated in Fig. 5B
  2. b The compound numbers represent the main peaks as indicated in Fig. 5D-G
  3. c The molecular formula and the compounds were deduced based on searching the m/z and MS/MS spectra of negative modes in “METLIN” database
  4. HHDP—Hexahydroxydiphenoyl unit
  5. Rt—Retention time
  6. [M-H]—Molecular ion