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Fig. 5 | BMC Plant Biology

Fig. 5

From: In planta high levels of hydrolysable tannins inhibit peroxidase mediated anthocyanin degradation and maintain abaxially red leaves of Excoecaria Cochinchinensis

Fig. 5

Isolation of natural POD inhibitors from Excoecaria leaves. A Separation of phenolic compounds from the Excoecaria leaf by Sephadex LH 20 column chromatography. Fifteen fractions were obtained based on the absorbance peaks at 280 nm of the chromatography profile. B The inhibition of the phenolic fractions on POD activity. The inhibition ratio on the POD activity indicated in Fig. 4G was measured by the addition of 100 μM GAE (gallic acid equivalent) of each fraction (A) to the activity assay reaction as described in Fig. 4C. C IC50 values of the fractions on POD. The half maximal inhibitory concentration (IC50) values of the fractions (F1, F2, F13, F14), which showed more than 50% inhibition on the POD activity as indicated in (B) and regarded to be of high inhibitory efficacy, were measured with gallic acid as a reference. D-G UPLC-DAD profiles of the high inhibition fractions represented by absorbance at 280 nm. The high inhibition fractions from Excoecaria leaves as indicated in (C) were respectively analyzed by UPLC-DAD

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