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Fig. 5 | BMC Plant Biology

Fig. 5

From: Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance

Fig. 5

Identification of hub genes in co-expression network and their expression levels under drought stress. a Eight hub genes and their connectivity with co-expressed genes in the network. The co-expression network was analyzed using WGCNA software and the graphic network was created by Cytoscape software. This co-expression network was constructed from 464 genes from six co-expression modules (Additional file 2: Figure S5). b Expression confirmation of eight Hub genes using qRT-PCR. Each sample was conducted three biological replicates and four technical replicates. FPKM values of eight Hub genes from RNA-Sequencing at four time points were shown as grey columns, and relative expression of target genes by qRT-PCR were shown as red lines with standard error. Transcript level at control (0 h) was set to 1. Details of the 464 co-expressed genes was listed in Additional file 6

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