Fig. 9

Identification of DNA and histone demethylation and expression of OsZIP1 in the wild-type (WT) and epigenetic modified mutant plants exposed to Cd. DNA methylation (a, d, g and j), H3K9me2 mark (b, e, h and K) and transcript levels (c, f, i and l) of OsZIP1 in ossdg714, osmet1, osrdr2i-6, osdrum2 and osros1 mutants and their wild-types with or without Cd exposure. Two weeks-old young rice plants were grown in the nutrient solution supplemented with 0 and 80 μM Cd for 4 d. The rice Actin1 was used as a negative control. Anti-H3 was used as an internal reference in the ChIP-qPCR assay. Vertical bars represent standard deviation. Asterisks indicate that the mean values of three replicates are significantly different between the wild-type and mutants (p < 0.05)