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Fig. 2 | BMC Plant Biology

Fig. 2

From: OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice

Fig. 2

Subcellular localization of OsZIP1-GFP fusion proteins with plasma membrane (PM) and endoplasmic reticulum (ER) markers in rice mesophyll protoplast (a-h) and tobacco leaf epidermal cells (i-k) detected by Confocal images under normal metal supply. a OsZIP1-GFP. b PM marker PIP2A-RFP. c Bright field. d Merged images of a and b. e OsZIP1-GFP. f ER marker RFP-KDEL. g Bright field. h Merged images of e and f. i OsZIP1-GFP. j ER marker RFP-KDEL. k Merged images of i and j. l OsZIP1-GFP. m PM marker PIP2A-RFP. n Merged images of l and m. Arrows indicate the part of the ER or PM areas where OsZIP1-GFP fusion images were overlapped with the corresponding markers

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