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Fig. 3 | BMC Plant Biology

Fig. 3

From: A maize polygalacturonase functions as a suppressor of programmed cell death in plants

Fig. 3

ZmPGH1 protein suppresses NB-LRR protein-induced HR in N. benthamiana. (a) The HR induced by the transient expression of Rp1-D21-HA and the transient co-expression of HCT1806-EGFP, ZmPGH1-EYFP, or GUS-EYFP with Rp1-D21-HA. The picture was taken at 3 day-post-infiltration and this N. benthamiana leaf was bleached with ethanol to make HR more obvious; (b, d) Percentages of infiltration sites showing the HR induced by the co-expression of HCT1806-EGFP, ZmPGH1-EYFP or GUS-EYFP with Rp1-D21-HA (b) or the co-expression of ZmPGH1-EYFP or Gus-EYFP with RPM1(D505V) (d). The sites were scored each day post infiltration (dpi); (c, e) Western blot experiments were performed to detect transiently expressed proteins in N. benthamiana leaves. Total proteins were extracted from the infiltrated leaves at 40 h-post-infiltration (c, Rp1-D21) or 20 h-post-infiltration (E, RPM1). Anti-HA antibody was used to detect Rp1-D21-HA protein. Anti-GFP was used to detect HCT1806-EGFP, ZmPGH1-EYFP and Gus-EYFP proteins. Equal loading was shown by the red bands of Ponceau S-stained RuBisCO protein. All the experiments shown in this figure were performed at least two times with similar results. . Significant differences from GUS-EYFP are indicated at * p < 0.1 ** p < 0.05 ***p < 0.01 ****p < 0.001. Significance determined by 2-way ANOVA

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