Fig. 1

The mu1029380 transposon inserted at the upstream of ZmPGH1 gene causes enhanced expression. (a) The genes near the SNP at 121,288,617 bp on chromosome 7 (based on the maize B73 genome v2 at maizegdb.org), which was associated with variation in Rp1-D21-induced HR (Olukolu, Wang et al. 2014). Arrows indicate the direction and length of transcription of the genes; (b) The position of mu1029380 insertion relative to ZmPGH1 gene. White boxes indicate the UTRs. Black boxes indicate the exons. The polylines indicate the introns. The scale bar of this figure is 100 bp; (c) By qRT-PCR, we found the expression of ZmPGH1 gene was enhanced in four F₂ segregants from the UFMu-02561 family homozygous for the mu1029380 insertion (here termed ZmPGH1OE plants) compared to siblings homozygous for the absence of the insertion (termed wild type plants). Figure shows the result of one biological replication pooled from four plants. Three technical replications were performed for each biological replication. Error bars represent standard deviation. All replications demonstrated at least 1.6 fold enhanced expression of ZmPGH1 gene in ZmPGH1OE plants. Significant difference from wild type at * p < 0.1 ** p < 0.05 ***p < 0.01 ****p < 0.001. Significance determined by T-test