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Table 5 Descriptive statistics of the B. thunbergii cv. ‘Kobold’ reference-guided transcriptome assembly

From: Mapping non-host resistance to the stem rust pathogen in an interspecific barberry hybrid

Trinity reference-based assembly results

Number of transcripts

122,872

Total length (bp)

189,291,041

Mean length (bp)

1541

Number of ORFs (%)

55,186 (44.28%)

Transcript length N50 (bp)

1991

GC Content

40.0%

TransRate results

TransRate score

0.403

TransRate optimal score

0.427

TransRate cutoff

0.037

Number of good contigs (%)

120,972 (98.5%)

BUSCO results

Complete (%)

1286 (89.3%)

Complete and single-copy (%)

651 (45.2%)

Complete and duplicated (%)

635 (44.1%)

Fragmented (%)

47 (3.3%)

Missing (%)

107 (7.4%)