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Table 4 Summary statistics of the B. thunbergii cv. ‘Kobold’ genome assembly, by stage

From: Mapping non-host resistance to the stem rust pathogen in an interspecific barberry hybrid

Variables

FALCON-Unzip

Final assemblya

Hi-C scaffoldingb

Primary

contigs

Haplotigs

Primary

contigs

Haplotigs

Number of contigs

4671

7144

2698

8790

14

Total length (Gbp)

1.36

0.88

1.23

0.99

1.20

Longest (Mbp)

8.60

1.49

8.60

1.49

99.76

Shortest (bp)

8581

561

20,469

561

54.72

>  100 kbp (%)

2551 (54.6)

2836 (39.7)

2229 (82.6)

3126 (35.6)

14 (100)

>  1 Mbp (%)

289 (6.2)

9 (0.1)

289 (10.7)

9 (0.1)

14 (100)

Mean length (Mbp)

0.29

0.12

0.46

0.11

85.40

N50 length (Mbp)

0.67

0.21

0.76

0.19

88.62

GC content (%)

37.6

37.7

37.7

37.7

37.66

  1. a After application of the Purge Haplotigs pipeline [61] and manual contig curation (i.e. chimera breaking and haplotig re-assignment)
  2. b All statistics for the Hi-C assembly refer to scaffolds rather than contigs. For details of individual pseudo-molecules, see Additional file 2: Table S2