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Table 4 List of Triticum aestivum lines used to set up the screening method and subsequent comparison

From: Outlook for coeliac disease patients: towards bread wheat with hypoimmunogenic gluten by gene editing of α- and γ-gliadin gene families

Triticum aestivum

Lines

Description

Cultivar 'Chinese Spring' (CS)

WT

Control

1AS-1

Kansas Deletion lines missing part of Chr 1 short arm that contain Gli-1 and/or Gli-3 loci where γ- and/or ω-gliadin genes are located

1AS-3

1BS-9

1BS-10

1DS-1

1DS-5

6AS-1

Kansas Deletion lines missing part of Chr 6 short arm that contain Gli-2 loci where α-gliadin genes are located

6BS-4/5BS-2

6DS-4

6DS-4/1BS-19

N1AT1D = 1DDB

Nullisomic-tetrasomic lines with one homoeologous pair of Chr 1 that contain Gli-1 and/or Gli-3 loci where γ- and/or ω-gliadin genes are located substituted by another homoeologous pair of Chr 1

N1AT1B = 1BBD

N1DT1A = 1AAB

N1DT1B = 1BBA

N1BT1D = 1DDA

N1BT1A = 1AAD

N6AT6D = 6DDB

Nullisomic-tetrasomic lines with one homoeologous pair of Chr 6 that contain Gli-2 loci where α-gliadin genes are located substituted by another homoeologous pair of Chr 6

N6AT6B = 6BBD

N6DT6A = 6AAB

N6DT6B = 6BBA

N6BT6D = 6DDA

Cultivar 'Paragon'

WT

Control

Line P3-47 to P6-79 and

360 γ-irradiated lines M4 generation

Line P10-19 to P10-96