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Table 3 Summary of T1 Fielder-CRISPR grains, and associated T0 plants, with modified gliadin protein profile

From: Outlook for coeliac disease patients: towards bread wheat with hypoimmunogenic gluten by gene editing of α- and γ-gliadin gene families

CRISPR/Cas9 constructs T0 plants expressing Cas9 T1 grains tested on Acid-PAGE T0 plants giving T1 mutant grains with clear changes T1 grains with clear changes in gliadin profile T0 plants giving T1 mutant grains with any changes T1 grains with any changes in gliadin profile
Construct α1_sgRNA
sgRNA_α87
38 325 3 (7.9%) 4 (1.2%) 3 (7.9%) 8 (2.5%)
Construct α2_sgRNA
sgRNA_α213 + sgRNA_α324
12 288 7 (58.3%) 13 (4.5%) 7 (58.3%) 19 (6.6%)
Construct γ3_sgRNA
sgRNA_γ86 + sgRNA_γ272 + sgRNA_γ603
35 280 10 (28.6%) 10 (3.6%) 15 (42.9%) 23 (8.2%)
Construct α2γ3_sgRNA
sgRNA_α213 + sgRNA_α324 + sgRNA_γ86 + sgRNA_γ272 + sgRNA_γ603
32 256 3 (9.4%) 11 (4.3%) 11 (34.4%) 20 (7.8%)
TOTAL 117 1149 23 (19.6%) 38 (3.3%) 36 (30.8%) 70 (6.1%)
  1. The plants and associated T1 grains tested on Acid-PAGE, as well as the number of T0 plants giving T1 mutant grains with “clear changes” or “any changes” in Acid-PAGE profiles, and the number of T1 mutant grains showing “clear changes” and “any changes”. For each construct mutations were observed. Mutations occurring in both the targeted and non-targeted gliadin families were included in “any changes” here.